SEQUENCE_ID=Pick primer and oligo
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_LOWERCASE_MASKING=0
PRIMER_TASK=generic
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 13974, GC content failed 3, low tm 2664, high tm 7479, long poly-x seq 44, ok 3784
PRIMER_RIGHT_EXPLAIN=considered 13974, GC content failed 3, low tm 2880, high tm 7234, long poly-x seq 44, ok 3813
PRIMER_INTERNAL_EXPLAIN=considered 14749, GC content failed 3, low tm 2973, high tm 7694, long poly-x seq 44, ok 4035
PRIMER_PAIR_EXPLAIN=considered 32, unacceptable product size 29, ok 3
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_INTERNAL_0_PENALTY=0.187324
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa
PRIMER_LEFT_0=198,20
PRIMER_RIGHT_0=315,20
PRIMER_INTERNAL_0=250,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_INTERNAL_0_TM=60.187
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=118
PRIMER_PAIR_0_PRODUCT_TM=74.3
=
SEQUENCE_ID=Excluded regions test overlapping binding sites, no conflict
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
SEQUENCE_EXCLUDED_REGION=220,70
SEQUENCE_INTERNAL_EXCLUDED_REGION=1,240 280,1200
PRIMER_LEFT_EXPLAIN=considered 13155, overlap excluded region 96, GC content failed 3, low tm 2471, high tm 7012, high any compl 18, high end compl 226, long poly-x seq 44, ok 3285
PRIMER_RIGHT_EXPLAIN=considered 13155, overlap excluded region 96, GC content failed 3, low tm 2687, high tm 6767, long poly-x seq 44, ok 3558
PRIMER_INTERNAL_EXPLAIN=considered 1906, overlap excluded region 1476, low tm 175, high tm 121, ok 134
PRIMER_PAIR_EXPLAIN=considered 6696, unacceptable product size 6058, high end compl 65, no internal oligo 572, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_INTERNAL_0_PENALTY=0.187324
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa
PRIMER_LEFT_0=198,20
PRIMER_RIGHT_0=315,20
PRIMER_INTERNAL_0=250,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_INTERNAL_0_TM=60.187
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=118
PRIMER_PAIR_0_PRODUCT_TM=74.3
=
SEQUENCE_ID=Internal exclusion shifting internal oligo
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
SEQUENCE_EXCLUDED_REGION=220,70
SEQUENCE_INTERNAL_EXCLUDED_REGION=1,500
PRIMER_LEFT_EXPLAIN=considered 13155, overlap excluded region 96, GC content failed 3, low tm 2471, high tm 7012, long poly-x seq 44, ok 3529
PRIMER_RIGHT_EXPLAIN=considered 13155, overlap excluded region 96, GC content failed 3, low tm 2687, high tm 6767, long poly-x seq 44, ok 3558
PRIMER_INTERNAL_EXPLAIN=considered 10249, overlap excluded region 510, GC content failed 3, low tm 2094, high tm 4964, long poly-x seq 44, ok 2634
PRIMER_PAIR_EXPLAIN=considered 39, unacceptable product size 32, high end compl 2, no internal oligo 3, ok 2
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.032388
PRIMER_LEFT_0_PENALTY=0.032351
PRIMER_RIGHT_0_PENALTY=0.000037
PRIMER_INTERNAL_0_PENALTY=0.077340
PRIMER_LEFT_0_SEQUENCE=gacacacttgctgctttcca
PRIMER_RIGHT_0_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_INTERNAL_0_SEQUENCE=catcttcggataaccgcatt
PRIMER_LEFT_0=609,20
PRIMER_RIGHT_0=868,20
PRIMER_INTERNAL_0=801,20
PRIMER_LEFT_0_TM=60.032
PRIMER_RIGHT_0_TM=60.000
PRIMER_INTERNAL_0_TM=59.923
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=9.8000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=260
PRIMER_PAIR_0_PRODUCT_TM=78.9
=
SEQUENCE_ID=Pick 10x tripletts
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_NUM_RETURN=10
PRIMER_LEFT_EXPLAIN=considered 13974, GC content failed 3, low tm 2664, high tm 7479, long poly-x seq 44, ok 3784
PRIMER_RIGHT_EXPLAIN=considered 13974, GC content failed 3, low tm 2880, high tm 7234, long poly-x seq 44, ok 3813
PRIMER_INTERNAL_EXPLAIN=considered 14749, GC content failed 3, low tm 2973, high tm 7694, long poly-x seq 44, ok 4035
PRIMER_PAIR_EXPLAIN=considered 86, unacceptable product size 71, high end compl 3, ok 12
PRIMER_LEFT_NUM_RETURNED=10
PRIMER_RIGHT_NUM_RETURNED=10
PRIMER_INTERNAL_NUM_RETURNED=10
PRIMER_PAIR_NUM_RETURNED=10
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_INTERNAL_0_PENALTY=0.187324
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa
PRIMER_LEFT_0=198,20
PRIMER_RIGHT_0=315,20
PRIMER_INTERNAL_0=250,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_INTERNAL_0_TM=60.187
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=118
PRIMER_PAIR_0_PRODUCT_TM=74.3
PRIMER_PAIR_1_PENALTY=0.020508
PRIMER_LEFT_1_PENALTY=0.014419
PRIMER_RIGHT_1_PENALTY=0.006089
PRIMER_INTERNAL_1_PENALTY=0.006937
PRIMER_LEFT_1_SEQUENCE=ttctctggacacagctccct
PRIMER_RIGHT_1_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_1_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_LEFT_1=39,20
PRIMER_RIGHT_1=315,20
PRIMER_INTERNAL_1=198,20
PRIMER_LEFT_1_TM=59.986
PRIMER_RIGHT_1_TM=60.006
PRIMER_INTERNAL_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=277
PRIMER_PAIR_1_PRODUCT_TM=78.6
PRIMER_PAIR_2_PENALTY=0.020508
PRIMER_LEFT_2_PENALTY=0.014419
PRIMER_RIGHT_2_PENALTY=0.006089
PRIMER_INTERNAL_2_PENALTY=0.006937
PRIMER_LEFT_2_SEQUENCE=cttctctggacacagctccc
PRIMER_RIGHT_2_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_2_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_LEFT_2=38,20
PRIMER_RIGHT_2=315,20
PRIMER_INTERNAL_2=198,20
PRIMER_LEFT_2_TM=59.986
PRIMER_RIGHT_2_TM=60.006
PRIMER_INTERNAL_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=278
PRIMER_PAIR_2_PRODUCT_TM=78.7
PRIMER_PAIR_3_PENALTY=0.032388
PRIMER_LEFT_3_PENALTY=0.032351
PRIMER_RIGHT_3_PENALTY=0.000037
PRIMER_INTERNAL_3_PENALTY=0.077340
PRIMER_LEFT_3_SEQUENCE=gacacacttgctgctttcca
PRIMER_RIGHT_3_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_INTERNAL_3_SEQUENCE=catcttcggataaccgcatt
PRIMER_LEFT_3=609,20
PRIMER_RIGHT_3=868,20
PRIMER_INTERNAL_3=801,20
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=60.000
PRIMER_INTERNAL_3_TM=59.923
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=260
PRIMER_PAIR_3_PRODUCT_TM=78.9
PRIMER_PAIR_4_PENALTY=0.037132
PRIMER_LEFT_4_PENALTY=0.031043
PRIMER_RIGHT_4_PENALTY=0.006089
PRIMER_INTERNAL_4_PENALTY=0.006937
PRIMER_LEFT_4_SEQUENCE=actggtctcccatcgtcaac
PRIMER_RIGHT_4_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_4_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_LEFT_4=106,20
PRIMER_RIGHT_4=315,20
PRIMER_INTERNAL_4=198,20
PRIMER_LEFT_4_TM=59.969
PRIMER_RIGHT_4_TM=60.006
PRIMER_INTERNAL_4_TM=59.993
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=210
PRIMER_PAIR_4_PRODUCT_TM=77.4
PRIMER_PAIR_5_PENALTY=0.037980
PRIMER_LEFT_5_PENALTY=0.031043
PRIMER_RIGHT_5_PENALTY=0.006937
PRIMER_INTERNAL_5_PENALTY=0.362989
PRIMER_LEFT_5_SEQUENCE=actggtctcccatcgtcaac
PRIMER_RIGHT_5_SEQUENCE=ggactgagctgttcgaggac
PRIMER_INTERNAL_5_SEQUENCE=cccagatgactttcaccacc
PRIMER_LEFT_5=106,20
PRIMER_RIGHT_5=217,20
PRIMER_INTERNAL_5=146,20
PRIMER_LEFT_5_TM=59.969
PRIMER_RIGHT_5_TM=59.993
PRIMER_INTERNAL_5_TM=60.363
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=60.000
PRIMER_INTERNAL_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_INTERNAL_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_INTERNAL_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=112
PRIMER_PAIR_5_PRODUCT_TM=76.5
PRIMER_PAIR_6_PENALTY=0.048625
PRIMER_LEFT_6_PENALTY=0.048588
PRIMER_RIGHT_6_PENALTY=0.000037
PRIMER_INTERNAL_6_PENALTY=0.077340
PRIMER_LEFT_6_SEQUENCE=cgacaaccaaaacgacacac
PRIMER_RIGHT_6_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_INTERNAL_6_SEQUENCE=catcttcggataaccgcatt
PRIMER_LEFT_6=596,20
PRIMER_RIGHT_6=868,20
PRIMER_INTERNAL_6=801,20
PRIMER_LEFT_6_TM=60.049
PRIMER_RIGHT_6_TM=60.000
PRIMER_INTERNAL_6_TM=59.923
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_INTERNAL_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_INTERNAL_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_INTERNAL_6_SELF_END=2.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=9.8000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=273
PRIMER_PAIR_6_PRODUCT_TM=78.9
PRIMER_PAIR_7_PENALTY=0.048625
PRIMER_LEFT_7_PENALTY=0.048588
PRIMER_RIGHT_7_PENALTY=0.000037
PRIMER_INTERNAL_7_PENALTY=0.077340
PRIMER_LEFT_7_SEQUENCE=acgacaaccaaaacgacaca
PRIMER_RIGHT_7_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_INTERNAL_7_SEQUENCE=catcttcggataaccgcatt
PRIMER_LEFT_7=595,20
PRIMER_RIGHT_7=868,20
PRIMER_INTERNAL_7=801,20
PRIMER_LEFT_7_TM=60.049
PRIMER_RIGHT_7_TM=60.000
PRIMER_INTERNAL_7_TM=59.923
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_INTERNAL_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_INTERNAL_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_INTERNAL_7_SELF_END=2.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=9.8000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=274
PRIMER_PAIR_7_PRODUCT_TM=78.8
PRIMER_PAIR_8_PENALTY=0.050921
PRIMER_LEFT_8_PENALTY=0.044832
PRIMER_RIGHT_8_PENALTY=0.006089
PRIMER_INTERNAL_8_PENALTY=0.006937
PRIMER_LEFT_8_SEQUENCE=cgaggattatggttgcacct
PRIMER_RIGHT_8_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_8_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_LEFT_8=80,20
PRIMER_RIGHT_8=315,20
PRIMER_INTERNAL_8=198,20
PRIMER_LEFT_8_TM=59.955
PRIMER_RIGHT_8_TM=60.006
PRIMER_INTERNAL_8_TM=59.993
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_INTERNAL_8_GC_PERCENT=60.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_INTERNAL_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_INTERNAL_8_SELF_END=2.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=236
PRIMER_PAIR_8_PRODUCT_TM=77.9
PRIMER_PAIR_9_PENALTY=0.051769
PRIMER_LEFT_9_PENALTY=0.044832
PRIMER_RIGHT_9_PENALTY=0.006937
PRIMER_INTERNAL_9_PENALTY=0.031043
PRIMER_LEFT_9_SEQUENCE=cgaggattatggttgcacct
PRIMER_RIGHT_9_SEQUENCE=ggactgagctgttcgaggac
PRIMER_INTERNAL_9_SEQUENCE=actggtctcccatcgtcaac
PRIMER_LEFT_9=80,20
PRIMER_RIGHT_9=217,20
PRIMER_INTERNAL_9=106,20
PRIMER_LEFT_9_TM=59.955
PRIMER_RIGHT_9_TM=59.993
PRIMER_INTERNAL_9_TM=59.969
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=60.000
PRIMER_INTERNAL_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_INTERNAL_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_INTERNAL_9_SELF_END=2.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=138
PRIMER_PAIR_9_PRODUCT_TM=77.5
=
SEQUENCE_ID=Pick 10x tripletts with one overlap positions for internal
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
SEQUENCE_INTERNAL_OVERLAP_JUNCTION_LIST=210
PRIMER_INTERNAL_MIN_THREE_PRIME_DISTANCE=3
PRIMER_NUM_RETURN=10
PRIMER_LEFT_EXPLAIN=considered 13974, GC content failed 3, low tm 2664, high tm 7479, high any compl 19, high end compl 228, long poly-x seq 44, ok 3537
PRIMER_RIGHT_EXPLAIN=considered 13974, GC content failed 3, low tm 2880, high tm 7234, long poly-x seq 44, ok 3813
PRIMER_INTERNAL_EXPLAIN=considered 14749, GC content failed 3, low tm 2973, high tm 7694, long poly-x seq 44, no overlap of required point 3981, ok 54
PRIMER_PAIR_EXPLAIN=considered 7595, unacceptable product size 6913, high end compl 72, no internal oligo 601, ok 9
PRIMER_LEFT_NUM_RETURNED=6
PRIMER_RIGHT_NUM_RETURNED=6
PRIMER_INTERNAL_NUM_RETURNED=6
PRIMER_PAIR_NUM_RETURNED=6
PRIMER_PAIR_0_PENALTY=0.020508
PRIMER_LEFT_0_PENALTY=0.014419
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_INTERNAL_0_PENALTY=0.006937
PRIMER_LEFT_0_SEQUENCE=ttctctggacacagctccct
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_LEFT_0=39,20
PRIMER_RIGHT_0=315,20
PRIMER_INTERNAL_0=198,20
PRIMER_LEFT_0_TM=59.986
PRIMER_RIGHT_0_TM=60.006
PRIMER_INTERNAL_0_TM=59.993
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=277
PRIMER_PAIR_0_PRODUCT_TM=78.6
PRIMER_PAIR_1_PENALTY=0.037132
PRIMER_LEFT_1_PENALTY=0.031043
PRIMER_RIGHT_1_PENALTY=0.006089
PRIMER_INTERNAL_1_PENALTY=1.344683
PRIMER_LEFT_1_SEQUENCE=actggtctcccatcgtcaac
PRIMER_RIGHT_1_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_1_SEQUENCE=aactgtcctcgaacagctcag
PRIMER_LEFT_1=106,20
PRIMER_RIGHT_1=315,20
PRIMER_INTERNAL_1=194,21
PRIMER_LEFT_1_TM=59.969
PRIMER_RIGHT_1_TM=60.006
PRIMER_INTERNAL_1_TM=59.655
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=210
PRIMER_PAIR_1_PRODUCT_TM=77.4
PRIMER_PAIR_2_PENALTY=0.050921
PRIMER_LEFT_2_PENALTY=0.044832
PRIMER_RIGHT_2_PENALTY=0.006089
PRIMER_INTERNAL_2_PENALTY=1.387061
PRIMER_LEFT_2_SEQUENCE=cgaggattatggttgcacct
PRIMER_RIGHT_2_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_2_SEQUENCE=ctcgaacagctcagtccatct
PRIMER_LEFT_2=80,20
PRIMER_RIGHT_2=315,20
PRIMER_INTERNAL_2=201,21
PRIMER_LEFT_2_TM=59.955
PRIMER_RIGHT_2_TM=60.006
PRIMER_INTERNAL_2_TM=59.613
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=236
PRIMER_PAIR_2_PRODUCT_TM=77.9
PRIMER_PAIR_3_PENALTY=0.072351
PRIMER_LEFT_3_PENALTY=0.066262
PRIMER_RIGHT_3_PENALTY=0.006089
PRIMER_INTERNAL_3_PENALTY=2.276812
PRIMER_LEFT_3_SEQUENCE=gccatcaatgacctccactt
PRIMER_RIGHT_3_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_3_SEQUENCE=cagctcagtccatctatcacca
PRIMER_LEFT_3=21,20
PRIMER_RIGHT_3=315,20
PRIMER_INTERNAL_3=207,22
PRIMER_LEFT_3_TM=59.934
PRIMER_RIGHT_3_TM=60.006
PRIMER_INTERNAL_3_TM=60.277
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=295
PRIMER_PAIR_3_PRODUCT_TM=78.9
PRIMER_PAIR_4_PENALTY=0.117903
PRIMER_LEFT_4_PENALTY=0.111814
PRIMER_RIGHT_4_PENALTY=0.006089
PRIMER_INTERNAL_4_PENALTY=3.038666
PRIMER_LEFT_4_SEQUENCE=ctggtctcccatcgtcaact
PRIMER_RIGHT_4_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_4_SEQUENCE=cgaacagctcagtccatctatc
PRIMER_LEFT_4=107,20
PRIMER_RIGHT_4=315,20
PRIMER_INTERNAL_4=203,22
PRIMER_LEFT_4_TM=60.112
PRIMER_RIGHT_4_TM=60.006
PRIMER_INTERNAL_4_TM=58.961
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=209
PRIMER_PAIR_4_PRODUCT_TM=77.4
PRIMER_PAIR_5_PENALTY=0.134664
PRIMER_LEFT_5_PENALTY=0.014419
PRIMER_RIGHT_5_PENALTY=0.120245
PRIMER_INTERNAL_5_PENALTY=6.275098
PRIMER_LEFT_5_SEQUENCE=ttctctggacacagctccct
PRIMER_RIGHT_5_SEQUENCE=tcatcaccaacttcatgcgt
PRIMER_INTERNAL_5_SEQUENCE=agctcagtccatctatcaccacaa
PRIMER_LEFT_5=39,20
PRIMER_RIGHT_5=331,20
PRIMER_INTERNAL_5=208,24
PRIMER_LEFT_5_TM=59.986
PRIMER_RIGHT_5_TM=60.120
PRIMER_INTERNAL_5_TM=62.275
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_INTERNAL_5_GC_PERCENT=45.833
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_INTERNAL_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_INTERNAL_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=9.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=293
PRIMER_PAIR_5_PRODUCT_TM=78.4
=
SEQUENCE_ID=Pick 10x tripletts with two overlap positions for internal
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
SEQUENCE_INTERNAL_OVERLAP_JUNCTION_LIST=210 810
PRIMER_INTERNAL_MIN_THREE_PRIME_DISTANCE=3
PRIMER_NUM_RETURN=10
PRIMER_LEFT_EXPLAIN=considered 13974, GC content failed 3, low tm 2664, high tm 7479, high any compl 2, high end compl 129, long poly-x seq 44, ok 3653
PRIMER_RIGHT_EXPLAIN=considered 13974, GC content failed 3, low tm 2880, high tm 7234, high any compl 1, high end compl 5, long poly-x seq 44, ok 3807
PRIMER_INTERNAL_EXPLAIN=considered 14749, GC content failed 3, low tm 2973, high tm 7694, long poly-x seq 44, no overlap of required point 3943, ok 92
PRIMER_PAIR_EXPLAIN=considered 4472, unacceptable product size 4076, high end compl 24, no internal oligo 358, ok 14
PRIMER_LEFT_NUM_RETURNED=10
PRIMER_RIGHT_NUM_RETURNED=10
PRIMER_INTERNAL_NUM_RETURNED=10
PRIMER_PAIR_NUM_RETURNED=10
PRIMER_PAIR_0_PENALTY=0.020508
PRIMER_LEFT_0_PENALTY=0.014419
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_INTERNAL_0_PENALTY=0.006937
PRIMER_LEFT_0_SEQUENCE=ttctctggacacagctccct
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_LEFT_0=39,20
PRIMER_RIGHT_0=315,20
PRIMER_INTERNAL_0=198,20
PRIMER_LEFT_0_TM=59.986
PRIMER_RIGHT_0_TM=60.006
PRIMER_INTERNAL_0_TM=59.993
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=277
PRIMER_PAIR_0_PRODUCT_TM=78.6
PRIMER_PAIR_1_PENALTY=0.032388
PRIMER_LEFT_1_PENALTY=0.032351
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_INTERNAL_1_PENALTY=0.077340
PRIMER_LEFT_1_SEQUENCE=gacacacttgctgctttcca
PRIMER_RIGHT_1_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_INTERNAL_1_SEQUENCE=catcttcggataaccgcatt
PRIMER_LEFT_1=609,20
PRIMER_RIGHT_1=868,20
PRIMER_INTERNAL_1=801,20
PRIMER_LEFT_1_TM=60.032
PRIMER_RIGHT_1_TM=60.000
PRIMER_INTERNAL_1_TM=59.923
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=260
PRIMER_PAIR_1_PRODUCT_TM=78.9
PRIMER_PAIR_2_PENALTY=0.037132
PRIMER_LEFT_2_PENALTY=0.031043
PRIMER_RIGHT_2_PENALTY=0.006089
PRIMER_INTERNAL_2_PENALTY=1.344683
PRIMER_LEFT_2_SEQUENCE=actggtctcccatcgtcaac
PRIMER_RIGHT_2_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_2_SEQUENCE=aactgtcctcgaacagctcag
PRIMER_LEFT_2=106,20
PRIMER_RIGHT_2=315,20
PRIMER_INTERNAL_2=194,21
PRIMER_LEFT_2_TM=59.969
PRIMER_RIGHT_2_TM=60.006
PRIMER_INTERNAL_2_TM=59.655
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=210
PRIMER_PAIR_2_PRODUCT_TM=77.4
PRIMER_PAIR_3_PENALTY=0.048625
PRIMER_LEFT_3_PENALTY=0.048588
PRIMER_RIGHT_3_PENALTY=0.000037
PRIMER_INTERNAL_3_PENALTY=0.792166
PRIMER_LEFT_3_SEQUENCE=cgacaaccaaaacgacacac
PRIMER_RIGHT_3_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_INTERNAL_3_SEQUENCE=ttcggataaccgcattatcg
PRIMER_LEFT_3=596,20
PRIMER_RIGHT_3=868,20
PRIMER_INTERNAL_3=805,20
PRIMER_LEFT_3_TM=60.049
PRIMER_RIGHT_3_TM=60.000
PRIMER_INTERNAL_3_TM=60.792
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=273
PRIMER_PAIR_3_PRODUCT_TM=78.9
PRIMER_PAIR_4_PENALTY=0.050921
PRIMER_LEFT_4_PENALTY=0.044832
PRIMER_RIGHT_4_PENALTY=0.006089
PRIMER_INTERNAL_4_PENALTY=1.387061
PRIMER_LEFT_4_SEQUENCE=cgaggattatggttgcacct
PRIMER_RIGHT_4_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_4_SEQUENCE=ctcgaacagctcagtccatct
PRIMER_LEFT_4=80,20
PRIMER_RIGHT_4=315,20
PRIMER_INTERNAL_4=201,21
PRIMER_LEFT_4_TM=59.955
PRIMER_RIGHT_4_TM=60.006
PRIMER_INTERNAL_4_TM=59.613
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=236
PRIMER_PAIR_4_PRODUCT_TM=77.9
PRIMER_PAIR_5_PENALTY=0.072351
PRIMER_LEFT_5_PENALTY=0.066262
PRIMER_RIGHT_5_PENALTY=0.006089
PRIMER_INTERNAL_5_PENALTY=2.276812
PRIMER_LEFT_5_SEQUENCE=gccatcaatgacctccactt
PRIMER_RIGHT_5_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_5_SEQUENCE=cagctcagtccatctatcacca
PRIMER_LEFT_5=21,20
PRIMER_RIGHT_5=315,20
PRIMER_INTERNAL_5=207,22
PRIMER_LEFT_5_TM=59.934
PRIMER_RIGHT_5_TM=60.006
PRIMER_INTERNAL_5_TM=60.277
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_INTERNAL_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_INTERNAL_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_INTERNAL_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=295
PRIMER_PAIR_5_PRODUCT_TM=78.9
PRIMER_PAIR_6_PENALTY=0.117903
PRIMER_LEFT_6_PENALTY=0.111814
PRIMER_RIGHT_6_PENALTY=0.006089
PRIMER_INTERNAL_6_PENALTY=3.038666
PRIMER_LEFT_6_SEQUENCE=ctggtctcccatcgtcaact
PRIMER_RIGHT_6_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_6_SEQUENCE=cgaacagctcagtccatctatc
PRIMER_LEFT_6=107,20
PRIMER_RIGHT_6=315,20
PRIMER_INTERNAL_6=203,22
PRIMER_LEFT_6_TM=60.112
PRIMER_RIGHT_6_TM=60.006
PRIMER_INTERNAL_6_TM=58.961
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_INTERNAL_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_INTERNAL_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_INTERNAL_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=209
PRIMER_PAIR_6_PRODUCT_TM=77.4
PRIMER_PAIR_7_PENALTY=0.127796
PRIMER_LEFT_7_PENALTY=0.032351
PRIMER_RIGHT_7_PENALTY=0.095445
PRIMER_INTERNAL_7_PENALTY=0.853077
PRIMER_LEFT_7_SEQUENCE=gacacacttgctgctttcca
PRIMER_RIGHT_7_SEQUENCE=aagcggcaaaggaggttaat
PRIMER_INTERNAL_7_SEQUENCE=tccacatcttcggataaccg
PRIMER_LEFT_7=609,20
PRIMER_RIGHT_7=897,20
PRIMER_INTERNAL_7=797,20
PRIMER_LEFT_7_TM=60.032
PRIMER_RIGHT_7_TM=60.095
PRIMER_INTERNAL_7_TM=60.853
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_INTERNAL_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_INTERNAL_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_INTERNAL_7_SELF_END=6.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=6.2000
PRIMER_PAIR_7_COMPL_ANY=7.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=289
PRIMER_PAIR_7_PRODUCT_TM=79.4
PRIMER_PAIR_8_PENALTY=0.134664
PRIMER_LEFT_8_PENALTY=0.014419
PRIMER_RIGHT_8_PENALTY=0.120245
PRIMER_INTERNAL_8_PENALTY=6.275098
PRIMER_LEFT_8_SEQUENCE=ttctctggacacagctccct
PRIMER_RIGHT_8_SEQUENCE=tcatcaccaacttcatgcgt
PRIMER_INTERNAL_8_SEQUENCE=agctcagtccatctatcaccacaa
PRIMER_LEFT_8=39,20
PRIMER_RIGHT_8=331,20
PRIMER_INTERNAL_8=208,24
PRIMER_LEFT_8_TM=59.986
PRIMER_RIGHT_8_TM=60.120
PRIMER_INTERNAL_8_TM=62.275
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_INTERNAL_8_GC_PERCENT=45.833
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_INTERNAL_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_INTERNAL_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=9.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=293
PRIMER_PAIR_8_PRODUCT_TM=78.4
PRIMER_PAIR_9_PENALTY=0.262891
PRIMER_LEFT_9_PENALTY=0.262854
PRIMER_RIGHT_9_PENALTY=0.000037
PRIMER_INTERNAL_9_PENALTY=0.853077
PRIMER_LEFT_9_SEQUENCE=acttcacagccaatcaagcc
PRIMER_RIGHT_9_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_INTERNAL_9_SEQUENCE=cggtccacatcttcggataa
PRIMER_LEFT_9=667,20
PRIMER_RIGHT_9=868,20
PRIMER_INTERNAL_9=794,20
PRIMER_LEFT_9_TM=60.263
PRIMER_RIGHT_9_TM=60.000
PRIMER_INTERNAL_9_TM=60.853
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_INTERNAL_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_INTERNAL_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_INTERNAL_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=9.8000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=202
PRIMER_PAIR_9_PRODUCT_TM=78.9
=
